Bead Assisted Mass Spectrometry (BAMS): A High-Resolution, Immunoassay Platform for Multiplexed Biomarker Screening
(Oct 13, 2021 02:00 PM in Eastern Time, US and Canada)
The ability to quantitatively probe diverse panels of proteins and their post-translational modifications (PTMs) across multiple samples would aid a broad range of biological, biochemical, and pharmacological research efforts. A novel microarray-based immunoassay technology that can support highly multiplexed, targeted proteomic studies has been developed by Adeptrix. The technology, termed Affinity-Bead Assisted Mass Spectrometry (Affi-BAMS), combines immunoaffinity capture with mass spectrometry detection, and it enables the development of highly specific and customizable assay panels for simultaneous profiling of multiple proteins, PTMs, and proteoforms. The Affi-BAMS workflow uses a bead-based enrichment approach in which each bead contains a single, protein (or site) specific antibody. Multiplexing capability is achieved by combining Affi-BAMS beads that have different specificities. With Affi-BAMS, both intact proteins and protein fragments can be monitored for targeted proteomics applications.
Affi-BAMS reagents have been created for protein targets in the areas of oncology, neurology, epigenetics, virology, and food allergens. The presentation will introduce the Affi-BAMS workflow and highlight several application areas.