Reconstructed Tissues
At Krishgen Biosystems, we are proud to partner with EPISKIN, the global leader in tissue engineering, to offer cutting-edge reconstructed human tissue models. These innovative in vitro models are designed to closely replicate the structure and function of human tissues, providing researchers with ethical and reliable alternatives to traditional methods.
What Are Reconstructed Tissues?
Reconstructed tissues are advanced three-dimensional cell culture systems developed from human cells. They mimic the histological structure, functionality, and cellular interactions found in natural human tissues. By replicating the complexity of human biology, these models allow researchers to conduct studies in a controlled environment that provides highly predictive results for in vivo applications.
Benefits of Reconstructed Human Tissues:
Enhanced Physiological Relevance
Reconstructed human tissues more closely mimic human biology compared to animal models, leading to more reliable and predictive research results.
Improved Research Efficiency
These tissues can be readily available and standardized, allowing for faster and more consistent research processes.
Reduced Reliance on Animal Models
Reconstructed human tissues offer an ethical alternative to animal models, aligning with evolving research practices.
Reproducibility
Standardized manufacturing processes ensure consistency across batches, leading to reliable experimental outcomes.
Comprehensive Applications
Suitable for various studies, including toxicology, drug discovery, efficacy testing, and mechanistic research.
Available Models and Applications:
T-Skin Human Full Thickness Model
RHE Human Epidermis
RHE-LC Human Epidermal Model Langerhans cells
RHPE Pigmented Epidermis
Corneal Epithelium
Oesophageal Epithelium
Oral Epithelium
Gingival Epithelium
Bladder Epithelium
Vaginal Epithelium
More about our Principal Partner:
Episkin’s unique cell culture process allows to industrialize important volumes of in vitro models of reconstructed human tissues with well characterized histology, functionality and ultrastructure features. The production unit has been designed to deliver every week our clients with thousands of human tissues – a process optimized and perfected by Krishgen for India over the last decade.
Based on easy-to-use and validated protocols, Episkin tissue models allow industrial corporations to screen and measure with high precision, the irritation, penetration, metabolism, or efficacy of large number of formulations or actives, to eliminate those that are unsuitable, so that the final (in vivo or clinical) tests can be carried out on a selected few.
Looking for native, donor tissues instead? Explore products from other partners.
Compliance with OECD Guidelines and Relevant Models
Reconstructed human tissue models have gained widespread acceptance under OECD Test Guidelines, ensuring global validation for regulatory safety assessments. These guidelines define standard protocols for using in vitro methods to evaluate safety and efficacy, replacing the need for animal testing. Many of our models comply with these internationally recognized guidelines, providing confidence and reliability for your studies.
- OECD TG 431:
- Model: SkinEthic™ RHE
- Use this model to assess skin corrosion potential in compliance with the OECD standard for in vitro testing.
- OECD TG 439
- Model: SkinEthic™ RHE
- A validated alternative for skin irritation testing, ensuring high reproducibility and human relevance.
- OECD TG 492
- Model: SkinEthic™ HCE (Human Corneal Epithelium)
- This corneal model is ideal for identifying chemicals that could cause eye irritation, offering a reliable and ethical solution.
- OECD TG 498
- Model: RHE-LC (Reconstructed Human Epidermis with Langerhans Cells)
- Used for studying skin sensitization through immune response pathways.
Frequently Asked Questions
It depends on the test protocol: large cultures allow easiest application of small quantities of creams (2 mg/cm² for example).
Yes, the medium is the same, but growth medium has to be used for more than 48 hours of culture.
Always leave them at room temperature.
For whitening tests : we recommend to use phototype VI.
For pigmentation induced tests, we recommend to use phototype IV.
The kit consists of reconstructed epidermis units (0.38 cm² or 1.07 cm²) upon which products can be applied directly and their pharmaco-toxicological effects evaluated.
Each reconstructed skin unit consists of type I collagen matrix, representing the dermis, surfaced with a film of type IV collagen, upon which is laid a stratified and differentiated epidermis derived from human keratinocytes.
A wide variety including hydrosoluble liquids, oils, creams, gels and solids. In case you are unsure about how to conduct the testing using your sample type, get in touch with the Krishgen team for support.
Once we get the order at Krishgen, we forward it to (Episkin, France) and they start custom preparation of the tissues.
It takes between 3 to 4 weeks for the delivery of products. The Krishgen team can provide a timeline and tissue production schedule to you, so that you may better plan your testing.
- the RHE model is a 17-day cultured tissue model, so Episkin dispatches the shipment on 14th day of its culturing, and we will receive it on the 17th day in India.
- On the same day, we will dispatch the tissues to you.
- From the 17th day, you will get 7 days to perform the experiment (i.e. shelf life 7 days). This is longer than any other competitor in the world.
Prefer testing at our lab? Reach out to our services team to learn more.