Protein Purification and Other Contamination Detection ELISA
Protein A and Protein L are commonly used affinity ligands for the purification of antibodies and other proteins. Accurate quantification of these proteins is essential for optimizing purification processes and ensuring product quality.
Protein L was first isolated from the surface of bacterial species Peptostreptococcus magnus and and is used as ligand in chromatography media for capture of Ig and Ig fragments. It consists of 719 amino acid residues. Protein L binds to the variable region of the kappa light chain of the antibody, without interfering with the antigenbinding site. This allows Protein L to bind a wider range of Ig classes and subclasses than other antibody-binding proteins. In addition, Protein L also binds antibody fragments such as Fabs, single-chain variable fragments and domain antibodies which contain the kappa light chain.
Applications:
- Protein Purification: Monitor the efficiency of protein purification processes using Protein A or Protein L affinity chromatography.
- Quality Control: Ensure the purity and consistency of purified proteins.
- Process Development: Optimize purification protocols based on Protein A or Protein L levels.
Quantify Protein A and Protein L Ligands with Krishgen
Krishgen, a leading manufacturer of ELISA kits, offers highly sensitive and commercially validated Protein A and Protein L detection and quantification assays.
Why Choose Krishgen’s Protein A and Protein L ELISA Kits?
- High Sensitivity: Detect even low levels of Protein A and Protein L for precise quantification.
- Specificity: Ensure accurate measurement without interference from other proteins.
- Ease of Use: User-friendly format for efficient and reliable testing.
- Fast Results: Obtain results within a short timeframe.
Quantify Protein L Ligands with world’s leading provider Medicago
The Protein L Ligand Leakage ELISA kit was originally developed by Medicago on behalf of GE Healthcare (currently Cytiva) in the 2010s. Today, the kit is a listed standard Medicago product, available to purchase for any organization, e.g. for research purposes or for quality control of pharmaceuticals.
Medicago’s Protein L Ligand Leakage ELISA kit is a quantitative enzyme-linked immunosorbent assay designed to detect and quantify ligand leakage in eluates from Protein L affinity chromatography medium containing immunoglobulin (Ig) or Ig fragments.
Quantify CRM197 and Endonuclease Serratia Mascarens with Krishgen
To ensure the safety of recombinant-expressed biopharmaceuticals as well as vaccines, health authorities are demanding the close monitoring of impurities caused during purification processes or via media. Detection methods with high sensitivity and specificity provide accurate information about the level of contamination in the in-process or finished products.
Serum free media has become a very popular choice for cell culturing, however, it may still contain trace elements of albumins or other proteins.
Similarly, to provide accurate, reliable quantification of contaminants from the bio-process phase including purification, Krishgen offers validated ELISA. Often one of the only commercial ELISA available for many of these markers, they are in-stock and competitively priced.
