N-Zyme Scientifics
N-Zyme Scientifics was created in 2013 to initially produce and sell PNGase F PRIME™, a high performance version of Peptide-N-Glycosidase F. Use of this product can, for example, eliminate the need to denature tissue samples before glycan analysis, saving valuable time and money in addition to obtaining significantly better quality data. Currently, PNGase F PRIME™ can be purchased directly from our valued distributors located in North America, Europe and Asia.
Featured Products
- PNGase F PRIME™ GLYCOSIDASE (Liquid Format) – This enzyme is a proprietary mutant recombinant PNGase F, cloned from Flavobacterium meningosepticum and expressed and purified from E. coli. Excellent for use in high-end use applications especially those using or requiring UPLC/HPLC, Hydrophilic Interaction Chromatography (HILIC), and/or MALDI-Glycan Mass Spec Imaging. 1 Unit of enzyme will completely deglycosylate 10 µg of denatured RNase B following incubation for 10 minutes at 37°C. Impact on protein can be visualized by SDS-PAGE. Released glycans can be examined following labeling with the Waters RapiFluor-MS dye and analyzed by normal phase hydrophilic interaction chromatography (HILIC) using various HPLC/UPLC-based systems. Imaging of released glycans directly on tissue can be done following spraying of enzyme on tissue and incubation at 37°C for 1 hour. (At our standard concentration of 2mg/mL, one 50 µL vial can make 4 slides.) Glycans can be detected using instruments such as the Bruker Daltonics SolariX™ 7T Hybrid FTMS System, a Bruker Daltonics RapifleXTM MALDI Tissuetyper, or a Bruker Daltonics UltrafleXtreme MALDI-TOF/TOF mass spectrometer.
- PNGase F PRIME-LY™ GLYCOSIDASE (Lyophilized Format) – PNGase F PRIME-LY™ Glycosidase is the same enzyme as our standard liquid format PNGase F PRIME™ but now available in a lyophilized form, which is perfectly suitable for use in solution-based analyses. Performance characteristics and applications in which it can be used remain exactly the same as with our standard liquid format PNGase F PRIME™ enzyme. This enzyme specifically removes N-linked oligosaccharides from glycoproteins.
- PNGase F PRIME-LY™ ULTRA GLYCOSIDASE (Lyophilized Format) – PNGase F PRIME-LY™ ULTRA Glycosidase is optimized and specifically formulated for high resolution imaging in small structures or cells. This enzyme specifically removes N-linked oligosaccharides from glycoproteins. This is a high-performance enzyme that has been shown to require as few as 3,000 cells per replicate with 3-20% coefficient of variation to capture label-free N-glycan profiles (as shown in Angel et. al., J Proteome Res. 2019).
- Endo F3 PRIME-LY™ (Lyophilized Format) – Endo-β-N-acetylglucosaminidase F3 (Endo F3) cleaves in β(1-4) link in between the two core GlcNAcs of asparagine linked glycans. Endo F3 cleaves this link on core-fucosylated structures. Endo F3 can be applied to workflows alone or in conjunction with PNGase F PRIME™ to allow for structural characterization of core-fucosylated glycans in tissues while maintaining spatial localization. Endo F3 PRIME-LY™ comes in a lyophilized format that is perfectly suitable for use in solution-based analyses.
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