AAT Bioquest
AAT Bioquest has strived to innovate and push the boundaries of fluorescent dye technologies. Our unique, integrative approach to better, faster and smarter assay development draws from all fields of study, from chemistry to biology to informatics and more, leading to a wave of new generation photometric probes that utilize colorimetric, fluorimetric and luminescent technologies. With our team of passionate scientists and experts, AAT Bioquest seeks to address some of the most challenging problems faced in life science research, diagnostic R&D and drug discovery. Employing a strong network of experienced and professional distributors across the globe, we provide superior products and customized services to a diverse portfolio of clients spanning from small research institutions to Fortune 500 companies.
AAT Bioquest helping customers by manufacturing advanced custom and catalog fluorescent dye technologies, reagents and assay kits for: Flow Cytometers, Microplate Readers & Fluorescence Microscopes.
Featured Products
- Gelite™ Safe DNA Gel Stain – AAT Bioquest is committed to designing our products to be environment-friendly. It is part of how we enable our customers to make the world healthier, cleaner, and safer. Ethidium bromide (EtBr) has been commonly used as a DNA stain for many years. However, EtBr is harmful if swallowed and is very toxic if inhaled. EtBr has been shown to be mutagenic in various tests and is an aquatic toxin. SYBR® Safe was introduced as a safer alternative to EtBr and SYBR® Green, but unfortunately, it is much less sensitive than SYBR® Green. It only has sensitivity comparable to EtBr. Gelite™ Safe has been developed specifically to be less hazardous than EtBr for staining DNA in agarose and acrylamide gels with much higher sensitivity. Gelite™ Safe has greatly improved safety and uncompromised sensitivity. The exceptional sensitivity and strong DNA binding affinity of Gelite™ Safe allows DNA to be stained prior to or post electrophoresis without destaining. In addition to its superior binding properties, Gelite™ Safe is essentially non-fluorescent in the absence of nucleic acids showing very low background fluorescence. Upon binding to nucleic acids, Gelite™ Safe exhibits a considerable fluorescence enhancement by several orders of magnitude greater than that of EtBr. Gelite™ Safe was optimized to be compatible with various instruments, including UV and blue-light transiluminators, gel documentation systems, and laser scanners. It is the first single formulation that can be used in either the green or red channel at your preference. Unlike the membrane-permeant SYBR® Green, which is highly toxic to cells and the environment, the membrane-impermeant properties of Gelite™ Safe make it a much safer and noncytotoxic alternative. Furthermore, Ames testing has confirmed Gelite™ Safe to be significantly less mutagenic than EtBr and SYBR® Green, even at concentrations well above the working concentration used for gel staining. Ames mutagenicity test was performed in a dose-dependent manner for all test dyes pretreated with an S9 fraction from rat liver (SYBR® is a trademark of ThermoFisher).
| Catalog | Unit Size | Price (USD) | Additional Information |
|---|---|---|---|
| 17700 | 100 ul | $79 | 10,000X Water Solution |
| 17701 | 500 ul | $99 | 10,000X Water Solution |
| 17702 | 1 ml | $151 | 10,000X Water Solution |
| 17703 | 10 ml | $989 | 10,000X Water Solution |
| 17704 | 100 ul | $79 | 10,000X DMSO Solution |
| 17705 | 500 ul | $99 | 10,000X DMSO Solution |
| 17706 | 1 ml | $151 | 10,000X DMSO Solution |
| 17707 | 10 ml | $989 | 10,000X DMSO Solution |
| 17708 | 0.5 mL | $99 | GelRed Replacement, 10,000X in water |
| 17709 | 0.5 mL | $99 | GelRed Replacement, 10,000X in DMSO |
| 17710 | 0.5 mL | $99 | GelRed Alternative, 10,000X in water |
| 17711 | 0.5 mL | $99 | GelRed Alternative, 10,000X in DMSO |
Key features of single-wavelength calcium indicators
- Minimal background fluorescence at resting calcium levels
- Improved signal-to-background ratios
- Greatly enhanced cellular retention
- Cell-permeant formats for live cell calcium analysis (compatible with imaging, flow cytometry and microplate assays, such as HTS)
- Cell-impermeant formats for loading by microinjection, patch pipette, or pinocytic loading
- Ultraviolet (UV), violet and visible light-excitable formats
- Low-, medium- and high-affinity formats provides a sensitivity range to satisfy many applications
Power Styramide™ Signal Amplification (PSA™)
Advantages of PSA™ Imaging System
Power Styramide™ signal amplification resulting from the rapid catalyzation and covalent deposition of multiple Styramide™ substrates per HRP label translates to many practical benefits, including simplicity, enhanced sensitivity and specificity, and compatibility with other techniques. The higher reactivity of Styramide™ radicals permits more robust and expeditious labeling of Styramide™ at the HRP-target interaction site resulting in stronger signal intensity and better spatial resolution than TSA.
Key Features of PSA™:
- Ultra-sensitive detection of low-abundance targets, 100-fold greater than IHC, ICC, and IF methods
- High fluorescence intensity, 10 to 50-fold greater than tyramide
- Compatible with other fluorescent markers, staining techniques, and PSA™ imaging kits for multiplex analysis
- Higher reactivity of PSA™ radicals for faster results and equivalent sensitivity and resolution versus radiometric detection
- Conserve precious antibodies, PSA™ labeling achieves equivalent levels of sensitivity with a significant reduction in primary antibody
- PSA™ imaging kits are easy-to-use and provide sufficient reagents for 100 tests
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