Endotoxin Control and Testing Reagents
Lipopolysaccharide (LPS), also known as endotoxin, is a potent toxin found in the outer membrane of Gram-negative bacteria. LPS can contaminate biological materials, such as protein, peptide, or antibody samples, leading to serious consequences. Detecting and removing LPS is crucial for ensuring the safety and efficacy of biopharmaceuticals.
Sources of LPS Contamination:
- Gram-negative Bacteria: Contamination can occur during the manufacturing process if proper sterilization techniques are not followed.
- Cell Culture: Cell cultures can become contaminated with LPS if not maintained under sterile conditions.
- Environmental Factors: LPS can be introduced from the surrounding environment, such as dust, air, or water.
Prevention and Detection Strategies:
- Good Manufacturing Practices (GMP): Adhere to strict GMP guidelines to minimize the risk of LPS contamination.
- Sterilization Techniques: Employ effective sterilization methods, such as autoclaving or filtration, to eliminate LPS.
- Endotoxin Testing: Regularly test biological materials for LPS contamination using sensitive detection methods.
- LPS Removal: Use endotoxin removal filters or other techniques to reduce LPS levels in products.
Explore LPS Detection Solutions from AAT Bioquest:
Since its inception, AAT Bioquest has strived to innovate and push the boundaries of assay technology. More than a decade later, that commitment has stayed the same. Their unique, integrative approach to assay development draws from all fields of study, from chemistry to biology to informatics and more, leading to a wave of new generation photometric probes that utilize colorimetric, fluorimetric and luminescent technologies.
AAT Bioquest offers two Endotoxin Detection Tools:
- Fast and Convenient: Get results within 60 minutes with a simple and user-friendly format.
- High Sensitivity: Detect LPS as low as 0.005 EU/mL, ensuring product safety even at trace levels.
- Broad Specificity: Detects a wide range of endotoxin types from various Gram-negative bacteria.
- Ideal for: Routine endotoxin testing in biomanufacturing and quality control.
- Cost-Effective: Affordable option for reliable endotoxin detection.
- Easy-to-Use: Streamlined ELISA format for efficient and accurate testing.
- Moderate Sensitivity: Detects LPS as low as 0.002 EU/mL, suitable for various applications.
- Broad Specificity: Detects a wide range of endotoxins from different Gram-negative bacteria.
- Ideal for: Process development, research, and routine screening assays.
