Transfection Reagents

Efficient Nucleic Acid Delivery for Advanced Cell and Gene Therapy Applications

 

Transfection is a critical step in cell and gene therapy (CGT), enabling the precise introduction of nucleic acids into cells for gene editing, protein expression, and therapeutic applications. The choice of transfection reagent directly impacts efficiency, cell viability, scalability, and reproducibility—all essential for clinical and research-grade applications.

At Krishgen Biosystems, we offer a comprehensive selection of transfection solutions from our global partners, covering lipid-based, polymer-based, viral transduction enhancers, and electroporation systems—all optimized for CGT workflows.

Key Benefits of Our Transfection Reagents

  • Optimized for Cell & Gene Therapy – Ensures high efficiency, low cytotoxicity, and reproducibility in primary cells, iPSCs, and T cells.
  • Broad Compatibility – Suitable for plasmid DNA, mRNA, siRNA, CRISPR components, and viral vectors.
  • Scalability – Reagents validated for both research and GMP-compliant bioprocessing.
  • Regulatory-Ready Solutions – Available for clinical and commercial cell therapy manufacturing.

Brand & Quality Assurance

Our Trusted Partners

Sino Biological

High-Performance Transfection Reagents
✔ Sinofection® – A proprietary polymer-based transfection reagent optimized for low toxicity and high repeatability in various cell types.
✔ Validated for Large-Scale Use – Suitable for mammalian cell lines, stem cells, and primary cells.
✔ High Purity & Efficiency – Designed for gene therapy, viral vector production, and protein expression.
[🔗 Explore Sino Biological Transfection Products]

ABM (Applied Biological Materials)

High-Purity Transfection Kits
✔ DNAfectin™ Plus – A powerful lipid-based DNA transfection reagent compatible with a wide variety of cells.
✔ ViralEntry™ Enhancer – Boosts efficiency of viral vector delivery, improving transduction rates in CGT applications.
✔ Scalable & Reproducible – Supports high-throughput and clinical-grade research.
[🔗 Explore ABM Transfection Reagents]

Considerations for Selecting Transfection Reagents in CGT

When choosing transfection reagents for cell and gene therapy applications, consider the following factors:

    • Cell Type Specificity: Some reagents are optimized for specific cell types, including primary cells or stem cells.​
    • Transfection Efficiency: High efficiency is crucial for therapeutic applications to achieve the desired gene expression levels.​
    • Cytotoxicity: Low toxicity is essential to maintain cell viability, especially when scaling up for clinical applications.​
    • Scalability: Reagents should be suitable for large-scale manufacturing processes, adhering to Good Manufacturing Practice (GMP) standards.

Lipid-based transfection reagents function by forming liposomes or lipid nanoparticles (LNPs), encapsulating nucleic acids to facilitate their entry into cells. These reagents are widely used for plasmid DNA, mRNA, and siRNA delivery, ensuring high efficiency and minimal cytotoxicity.

📌 Available Products:

Sinofection® (Sino Biological)

Composition: Proprietary cationic polymer-lipid hybrid system

Key Features:
✔ Higher transfection efficiency than traditional liposome-based reagents
✔ Low cytotoxicity, ensuring high cell viability post-transfection
✔ Compatible with suspension and adherent cells, including stem cells and iPSCs
✔ Validated for large-scale use in gene therapy, viral vector production, and protein expression

Applications:
✔ Transient and stable transfection of plasmid DNA (pDNA) in mammalian cells
✔ CRISPR/Cas9 delivery for genome editing applications
✔ mRNA transfection for cell reprogramming and protein expression

🔗 View Sinofection® Transfection Reagent

Composition: Optimized lipid nanoparticle system for transfection

Key Features:
✔ Specifically designed for large plasmids (>10kb) and multi-plasmid transfection
✔ No optimization needed – pre-validated for multiple cell lines
✔ Compatible with both serum-free and serum-containing media

Applications:
✔ Gene delivery for CAR-T cell engineering
✔ High-yield transfection for difficult-to-transfect cell types
✔ Stem cell reprogramming and differentiation studies

🔗 View LP4K Transfection Reagent

Polymer-based transfection reagents are particularly useful for large-scale plasmid DNA transfection and viral vector production in bioproduction and therapeutic applications. These reagents condense nucleic acids into nanoparticles, enhancing cellular uptake and expression efficiency.

📌 Available Products:

Sinofection® (Sino Biological)

✔ Cationic polymer-based transfection reagent for mammalian cell lines
✔ Higher transfection efficiency with reduced toxicity compared to PEI-based reagents
✔ Validated for scalable gene therapy manufacturing applications

Applications:
✔ Plasmid DNA transfection for stable cell line generation
✔ Gene expression studies in primary and suspension cell cultures
✔ Large-scale viral vector production (AAV, lentivirus, adenovirus)

🔗 Explore Sinofection® Polymer Transfection Reagent

Viral transduction enhancers improve the efficiency of viral vector-mediated gene delivery, ensuring high viral uptake and expression in target cells. These enhancers reduce the need for high MOI, making viral gene therapy applications more efficient and cost-effective.

📌 Available Products:

Trans-Up™ (GenTarget)

Composition: Proprietary poly-cationic enhancer with amphiphilic molecules

Key Features:
✔ Boosts lentiviral transduction efficiency in primary cells and immune cells
✔ Enhances viral vector delivery with lower MOI, improving cost-effectiveness
✔ Increases stable transgene expression for gene therapy applications

Applications:
✔ Lentiviral transduction for CAR-T and gene-modified immune cells
✔ Stable transgene expression in stem cell differentiation
✔ Gene therapy applications requiring precise viral vector delivery

🔗 View Trans-Up™ Viral Transduction Enhancer

✔ Optimized for lentivirus, adenovirus, and AAV transduction
✔ Increases virus uptake efficiency by modifying the cell membrane for enhanced entry
✔ Compatible with hard-to-transduce cells like hematopoietic and neural stem cells

Applications:
✔ Viral vector delivery in gene-modified T cells (CAR-T, TCR-T)
✔ In vivo and ex vivo gene therapy studies
✔ High-efficiency viral transduction in neuronal and hematopoietic cell lines

🔗 View ViralEntry™ Enhancer

mRNA transfection is critical for mRNA vaccines, protein expression, and gene-editing applications. Unlike plasmid DNA transfection, mRNA does not require nuclear entry, making it an attractive tool for rapid protein production and transient gene editing.

📌 Available Products:

mRNA-In™ (ABM)

✔ Optimized for high-efficiency mRNA delivery
✔ Minimal cytotoxicity, ensuring high viability in primary cells
✔ Validated for mRNA vaccine development and CRISPR applications

Applications:
✔ mRNA-based cell therapy development
✔ Protein production in mammalian cells
✔ CRISPR/Cas9 RNP transfection for genome editing

🔗 View mRNA-In™ Transfection Reagent

Expert Support: Our specialists assist with transfection protocol optimization and troubleshooting.
Custom Solutions: Tailored transfection reagents for research, preclinical, and GMP applications.
Regulatory Documentation Available: CoA, MSDS, and sterility testing reports included.

📩 [Contact Us for Pricing & Availability]